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Figure 1.
Figure 1. Identification of the BCOR BCL6 Binding Domain
(A) BCOR fragments were purified as thioredoxin (Trx) fusion
proteins and assayed for binding to the BCL6 BTB domain by
native gel electrophoresis. The horizontal box indicates the
position of unbound BCL6 BTB. BCL6^BTB was loaded alone (lane
“−”) or as a mixture with the Trx-BCOR fragments described
in (B). Equal amounts of BCL6^BTB were loaded in all lanes.
(B) Schematic of the long-isoform BCOR protein, indicating the
fragments used in the binding assay in (A).
(C) Crystal
structure of the BCL6^BTB/BCOR^BBD complex. The BCL6^BTB dimer
is shown in cartoon representation with blue and pink subunits.
The two bound BCOR^BBD peptides are shown in bond representation
with green and yellow carbons, respectively.
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