Figure 1.
Fig. 1. Pol structure in
ternary complex with lesion containing DNA. (A) In the 3'dG
elongation complex, the cisplatin (magenta) is shown with the
platinum anomalous electron density contoured at 10 .
The primer and template strands of the DNA (brown) and the
Watson-Crick H-bonded dCTP (magenta) are depicted as sticks. The
two metal ions are shown as gray spheres. (B) View of the
1,2-d(GpG) cisplatin lesion superimposed with the simulated
annealed composit-omit density map contoured at 1.0 sigma. (C)
The catalytic residues in the active site. R73 orients the dCTP
for H-bonding with the 3'desoxyguanine of the lesion. (D)
Schematics of protein-DNA contacts representing the
pre-elongation, 3'dG elongation, and 5'dG elongation complex.
Direct hydrogen-bonds are indicated by solid lines. DNA contacts
with the symmetry-related molecule are not shown.
The above figure is reprinted
by permission from the AAAs:
Science
(2007,
318,
967-970)
copyright 2007.
structure in
ternary complex with lesion containing DNA. (A) In the 3'dG
elongation complex, the cisplatin (magenta) is shown with the
platinum anomalous electron density contoured at 10 
.
The primer and template strands of the DNA (brown) and the
Watson-Crick H-bonded dCTP (magenta) are depicted as sticks. The
two metal ions are shown as gray spheres. (B) View of the
1,2-d(GpG) cisplatin lesion superimposed with the simulated
annealed composit-omit density map contoured at 1.0 sigma. (C)
The catalytic residues in the active site. R73 orients the dCTP
for H-bonding with the 3'desoxyguanine of the lesion. (D)
Schematics of protein-DNA contacts representing the
pre-elongation, 3'dG elongation, and 5'dG elongation complex.
Direct hydrogen-bonds are indicated by solid lines. DNA contacts
with the symmetry-related molecule are not shown.