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Figure 1.
Figure 1. Flexibility of the C-terminal extension (CTE) of
sLb^proC51A. (a) Ensemble of ten sLb^pro NMR structures (shown
in stereo). Regions of regular secondary structure were used for
the coordinate overlay of the final NMR solution structures.
α-Helices are shown in green and β-strands in magenta. The
variability of the structures of the extended CTE region
demonstrates its structural flexibility and presumable lack of
defined structure. (b) ^15N T[2] transverse relaxation times in
ms of sLb^proC51A (blue) and Lb^proC51A (red). Relaxation times
were measured as described in Materials and Methods. The
unstructured CTE is reflected in its T[2] relaxation times being
substantially longer than the globular domain. The roughly
twofold difference in relaxation rates between the two species
implies that Lb^proC51A is a dimer, whereas sLb^proC51A is a
monomer.
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