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Figure 1.
Figure 1. Overview of the PKA RIα(91–379):C Holoenzyme
Complex
Top: domain organization of the catalytic and
regulatory subunits. The two red spheres indicate the
phosphorylation sites Thr197^C and Ser338^C in the catalytic
subunit.
(A and C) (A) shows a view of the inhibitor
sequence of the regulatory subunit bound to the active-site
cleft of the catalytic subunit. Boxed regions indicate
interaction sites between the R and C subunits at the active
site (site 1, left) and the αG helix (site 2, right). (C) shows
a 180° rotation of the view in (A). Boxed regions indicate
the interaction site at the activation loop (site 3, top) and
αH-αI loop (site 4, bottom). The regulatory subunit is shown
as a cartoon representation with domain A in dark teal, domain B
in cyan, the phosphate-binding cassette (PBC) in yellow, and the
αB/C helix and inhibitor site in dark red.
(B and D)
Surface representation of both subunits in the same view as in
(A) and (C), respectively. The catalytic subunit is bound to
AMP-PNP (black sticks) and Mn^2+ (blue spheres) with the small
lobe (light tan) and the large lobe (dark tan) in surface
rendering.
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