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Figure 1.
FIGURE 1. Defining the minimal fragment of Borealin
necessary to bind Survivin and the Survivin interaction surface.
A, aligned primary sequences of the Borealin N terminus. The
yellow bars indicate sequences either strongly predicted
(thicker bar) or weakly predicted (thinner bar) to form a
coiled-coil structure. Details are shown in supplemental Fig.
S1. Boundary residues for the truncation constructs are
indicated above the sequences. B, summary of fragment
copurification studies. Interactions were scored based on
whether Borealin could be detected readily by Coomassie Blue
staining (+), only by Western blotting ((+)), or by neither
method (-) (see "Experimental Procedures"). n.d., not
determined. C, inferred interaction surface (teal) of
Borealin-(20-78) mapped onto the previously reported dimeric
Survivin structure (Protein Data Bank code 1F3H) (4). The
putative interaction surface spans the homodimer interface.
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