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Figure 1.
Figure 1. Structures of Rsc4
(A) Domain organization of
Rsc4 (top) and crystallized constructs (bottom). Rsc4 structure
is the following: bromodomain 1 (cyan), bromodomain 2 (blue),
wing insertion (orange), and the binding region for RSC and the
RNA polymerases (green) ([Kasten et al., 2004] and [Soutourina
et al., 2006]). First and last ordered residues in the
crystallized constructs are indicated with arrows. Acetylated
lysine residues are indicated with a magenta dot (K14 of H3 and
K25 of Rsc4).
(B) Orthogonal views of Rsc4(36–340) ribbon
diagram. Secondary structures are labeled, and termini are
indicated N and C. The asparagine and two tyrosine side chains
from each bromodomain that are important for acetyl lysine
binding are colored yellow. The wing insertion is colored
orange.
(C) Rsc4 amino acid sequence with secondary
structures indicated above. Residues not ordered in the
Rsc4(36–340) structure are indicated with a dashed line. The
red squares indicate interface residues between BD1 (residues
36–162) and BD2 (residues 163–320). The magenta circle
indicates the site of acetylation (K25).
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