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Figure 1.
Fig. 1. Initiator/origin complex. (A) Schematic of oriC2,
highlighting sequences recognized by Orc1-1, Orc1-2, and Orc1-3
paralogs (purple, gray, and teal arrows, respectively). The
direction of the purple arrows reflects the 5'-to-3' convention
of the mORB consensus sequence; the C2- and C3-site arrows
indicate the relative orientations of these repeats. The dashed
box denotes the C3/mORB dual site used in the cocrystal
structure. DUE, DNA unwinding element. (B) A cartoon of Orc1-1
illustrates the relative orientation of the initiator's
subdomains, with the ISM of the AAA+ domain in purple. ADP,
black sticks; magnesium ions, magenta sphere. (C) Global
architecture of the Orc1-1/Orc1-3  DNA complex.
Protein, purple and teal; DNA, orange; ADP, blacksticks;
magnesium ions, magenta spheres. (D) Electrostatic surface
representations of Orc1-1 and Orc1-3. The yellow line demarcates
the boundary between protomers.
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