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Figure 3.
Figure 3. CTCF binding. A GST fusion protein of the zinc
finger domains of human CTCF (lane 3) bound to glutathione beads
was incubated with the BRK domains of CHD7. After repeated
washing steps, binding of the BRK domains was analysed by
SDS–PAGE followed by Coomassie staining of the gel (lane 4).
GST served as a negative control (lanes 5 and 6). For
comparison, the BRK input is shown in lane 1. The BRK domains
did not bind to glutathione beads (lane 2).
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