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Figure 1.
Figure 1. Overview of the Structure of Human MutSα
(A)
Ribbon diagram of the structure of a MutSα/ADP/G•T mispair
complex. Blue, MSH6; red, MSH2; green ribbon, DNA; yellow, ADP;
and green spheres, Mg^2+ ions. Positions of the ABC ATPase
domains and the two channels in MutSα are indicated. Long α
helices connecting clamp and ATPase domains in MSH2 and MSH6 are
colored orange and cyan, respectively.
(B) Orthogonal,
expanded view of the DNA binding domains of MutSα. DNA is shown
as sticks, colored by atom type, with the central G•T mispair
colored yellow.
(C) Expanded view of the upper channel in
MutSα, colored as in (A) and shown as ribbons and a transparent
surface. Disordered loops are shown as dashed lines with residue
numbers.
(D) The domain structure of MSH6. Center: domains
1–5 are colored blue, green, yellow, orange, and red,
respectively. Periphery: exploded view of each domain, labeled
and colored with blue-red “chainbows” from the N- to C
termini of the domain. Figures were generated with PyMOL
(DeLano, 2002).
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