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Figure 1.
Figure 1. NMR structures of CRKI, CRKII and pCRKII[1–228].
(a) Domain structures. Tyr221 is the cABL phosphorylation site.
(b–d) Superpositions of 20 lowest-energy structures of CRKI
SH2 (b, left), CRKI SH3 (b, right), CRKII (c), pCRKII[1–228]
SH2 (d, left) and pCRKII[1–228] nSH3 (d, right). Magenta, SH2
(residues 10–120); green, nSH3 (134–191); blue, cSH3
(238–293); yellow, ISC (220–237) (e) Ribbon representation
of CRKII (left) and pCRKII[1–228] (right), colored as in
b–d. In both structures, nSH3 is presented in the same
orientation; ligand-binding sites in SH2 and SH3 are circled
(dotted circle in CRKII represents putative binding site of
cSH3). In pCRKII, the inter-SH2-nSH3 linker (121–133) is
colored orange and the phosphorylation site (221–224) is cyan.
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