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Figure 1.
Figure 1. Structure of the Skp1-Fbw7-CycE^degC Complex
(A) Overall architecture of the complex, with the secondary
structure elements of Skp1, F box, and linker domains labeled.
Dotted lines indicate disordered regions.
(B) CycE^degC
binds across the narrow face of the Fbw7 β-propeller structure.
The eight Fbw7 blades and the strands for one blade are labeled.
(C) Sequence alignment of the cyclin E peptides used in
crystallization with other SCF^Fbw7 substrates. Arrow indicates
the type II β turn, cylinder the left-handed polyproline II
helix, dotted lines disordered regions, and crosses the residues
of CycE^degC and CycE^degC that contact Fbw7. The substrate
residues that match the structure-based degron motif (  -X-
-
-
-pT/S-P-P-X-pS/T,
with representing
a hydrophobic residue and X any amino acid) are highlighted in
yellow.
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