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Figure 1.
Figure 1 Structure of the ARF1:ArfBD complex. (A) A cartoon
diagram of the ARF1:ArfBD complex is shown in two distinct
orientations. ARF1 is shown in grey with the  1
helix and switch I region in light and dark blue, respectively,
the interswitch region in green and the switch II region in red.
The Mg.GTP ligand is shown as a grey stick model. ArfBD is shown
in white with its  5'
region ( 5'
strand plus 5'–
6'
loop) in pink, the 1'
helix in orange and the Cter
helix in yellow. Tyr999 and Ile1053 of ArfBD are shown as stick
models. The two adjacent contact areas of the ARF1:ArfBD complex
interface are delineated by black boxes on the right-hand view.
(B) Detailed view of the interface between the Cter
helix and the PH domain of ArfBD. (C–F) Detailed views of the
ARF1:ArfBD interface. The secondary structures are shown as
ribbons and the residues as sticks. Hydrogen bonds are indicated
by dashed lines. (C) The 5'
region of ArfBD (pink) lies between the interswitch (green) and
switch I (blue) regions of ARF1 centred on Tyr999. (D) The
network of water-mediated interactions made between Asp996 of
the 5'
region (in pink) of ArfBD and ARF1. (E) The switch I (blue)
region of ARF1 interacts with the 5'
region (pink) and the 1'
helix (orange) of ArfBD. (F) The Cter
helix (yellow) of ArfBD is grasped between the switch II (red)
and the interswitch/switch I (green/blue) regions of ARF1.
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