|
Figure 1.
Figure 1. The structure of the Mms2–Ubc13-Ub complex. (a)
Blue, Mms2; magenta, Ubc13; orange, the covalently attached
donor ubiquitin. TheC terminus of the donor ubiquitin, Gly76, is
covalently attached to active site residue Ser87 of Ubc13(C87S).
(b) Packing interactions between complexes in the crystal. One
Mms2–Ubc13(C87S)-Ub complex is drawn as a surface
representation (same coloring as in a) and an adjacent complex
as ribbons (turquoise, Mms2; red, Ubc13; green, ubiquitin), to
show packing in the crystal. The latter ubiquitin (green) binds
in the acceptor site of the Mms2 from an adjacent complex
(blue). Lys63 of this acceptor ubiquitin lies in the active site
near the ester linkage.
|
