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Figure 1.
Fig. 1. Overall structure of the 1:1 complex of GatDE and
tRNA^Gln[1] (molecules B, D, and F). The protein domains are
colored differently; in GatD, the N-terminal domain, AnsA-like
domain 1, and AnsA-like domain 2 are shown in light green, blue,
and red, respectively; whereas in GatE, the AspRS-like insertion
domain, cradle domain, helical domain, and Yqey-like tail domain
are colored violet, cyan, orange, and pink, respectively. In the
present structure, the Yqey-like tail domain is shown as
translucent because its side chains are disordered, despite the
fact that the main chain was traced in the electron density map.
The bound tRNA molecules are yellow. In GatE, His15, Glu157, and
Glu184, which coordinate to an essential Mg2+ ion (red), are
shown to highlight the Glu-tRNA^Gln kinase and amidotransferase
sites. Gln240, which recognizes A73 (red), and Asp463, which
recognizes G52 (red), are indicated. All figures of the
molecular models were prepared with the program CueMol
(www.cuemol.org/).
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