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Figure 1.
Fig. 1. Loop structures and sequences of WW domains. (a)
Backbone diagram of the loop 1 substructure in WT Pin WW
(residues S16–R21) [Protein Data Bank (PDB) ID code 1PIN]. (b)
Backbone diagram of the loop 1 substructure in WT FBP WW
(residues T13–K17) (PDB ID code 1E01). Backbone H-bonds are
indicated by black dotted lines. (c) Aligned sequences of the WT
Pin WW domain (variant 1) and loop 1 redesigned variants 2–9
and the redesigned and sequence-minimized FBP WW variants (10
and 11).  -strand residues are
colored blue, residues that were mutated or deleted upon loop 1
redesign are in red, and all other residues are in gray.
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