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Figure 1.
Figure 1. Genetic analysis of the interface between Ubc9 and
RanGAP1. (a) Stereo view of the Ubc9 active site in complex
with RanGAP1–SUMO^14 (PDB entry 1Z5S) in ribbon and solid-bond
representation, depicting Ubc9 residues selected for genetic
analysis. Residues are labeled and hydrogen-bonding interactions
indicated by dashed lines. Yellow, SUMO-1; pink, RanGAP1; blue,
Ubc9. Images generated with PyMOL^44. (b) Serial dilutions of S.
cerevisiae ubc9 cultures bearing wild-type (WT) UBC9 or
indicated ubc9 alleles containing single or double point
mutations were spotted on YPAD agar and tested for growth at 23
°C (top), 30 °C (middle) and 37 °C (bottom) (Note:
D127S allele was expressed under yeast endogenous promoter; see
text). (c) Western blotting analysis for Smt3 conjugates (top)
or Ubc9 (bottom) in strains containing indicated ubc9 alleles.
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