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Figure 1.
Figure 1. Structure of the Rabex-5 A20 ZnF domain and IUIM.
(a) The domain structures of Rabex-5 and the construct used in
this study. Magenta, ZnF domain; green, IUIM; HB, helical
bundle; Vps9, Vps9-homology domain; CC, coiled coil; PR,
proline-rich. (b) Rabex-5 (ribbon) contacts ubiquitin (orange
and blue surfaces) at two sites in the crystal lattice. (c)
Superposition of the four crystallographically independent
molecules of the A20 ZnF domain and IUIM. Green, P6[1] molecule;
pink, magenta and red, the three independent molecules in the
asymmetric unit of the C2 lattice. (d) The zinc-binding site in
the A20 ZnF domain. Orange, sulfurs of cysteine side chains; red
sphere, zinc. (e) Ribbon and stick representation showing
exposed hydrophobic side chains. (f) Surface of the A20 ZnF
domain and IUIM colored by residue type: green, hydrophobic;
red, acidic; blue, basic; white, uncharged polar; yellow,
cysteine.
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