Figure 1.
Figure 1. Exo70p is a long rod composed of contiguous -helical
bundles. (a) Ribbon diagram of Exo70p colored from blue at
its N terminus to red at its C terminus. Domains A-D are
indicated. (b) Exo70p colored according to electrostatic surface
potential (on a scale from -15 kT to +15 kT), where blue is
positive and red is negative. Exo70p is strikingly dipolar, with
the N terminus negatively and the C terminus positively charged.
The orientation on the left is as in a. Arrows indicate a
conserved patch of arginine and lysine residues at the extreme C
terminus of Exo70p (see Supplementary Fig. 2). (c) Sequence of
Exo70p. Secondary structure elements are labeled as in a;
helices that span domains are blue. Dotted lines represent
regions in the crystal structure that are disordered. This
figure was prepared using MolScript32 and GRASP
(http://trantor.bioc.columbia.edu/grasp/)33.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2005,
12,
1094-1100)
copyright 2005.
-helical
bundles. (a) Ribbon diagram of Exo70p colored from blue at
its N terminus to red at its C terminus. Domains A-D are
indicated. (b) Exo70p colored according to electrostatic surface
potential (on a scale from -15 kT to +15 kT), where blue is
positive and red is negative. Exo70p is strikingly dipolar, with
the N terminus negatively and the C terminus positively charged.
The orientation on the left is as in a. Arrows indicate a
conserved patch of arginine and lysine residues at the extreme C
terminus of Exo70p (see Supplementary Fig. 2). (c) Sequence of
Exo70p. Secondary structure elements are labeled as in a;
helices that span domains are blue. Dotted lines represent
regions in the crystal structure that are disordered. This
figure was prepared using MolScript32 and GRASP
(http://trantor.bioc.columbia.edu/grasp/)33.