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Figure 1.
(A) The crystal structure of an RT fragment-DNA-netropsin complex. The
asymmetric unit consists of one protein molecule, an 8 bp oligonucleotide duplex and one
netropsin molecule representing half of the symmetric complex. The dashed vertical line
divides the symmetrically equivalent halves of the 16 bp oligonucleotide. The DNA
oligonucleotide is shown in a red sticks model, and netropsin is shown in a purple CPK
model. RT is shown as a ribbon rendering with ß-strands in green, coils in yellow and
{alpha} -helices in blue except for the {alpha} D helix in magenta. Residues
Tyr-64, Asp-114, Leu-115, Arg-116 and Gly-191 that make contacts with the DNA are shown in
black ball-and-sticks models. (B) Schematic of the oligonucleotide duplex with the two
complementary strands (B and G) and the numbering scheme referred to in the text. Arrows
denote netropsin molecules oriented from guanidinium (tail) to amidinium end (head).
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