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Figure 1.
Figure 1. a: Itch-ww3 sequence. Two possible sites identified
by NetPhos 2.0 phosphorylation prediction server are indicated
in red. The immediate amino acid environment of Thr30 is typical
of a phosphorylation site for Casein kinase II, CK2, whereas
protein kinase A, PKA is predicted to phosphorylate both Thr30
and Ser16. b: A ribbon representation of Itch-ww3 domain showing
the side chains of conserved aromatic residues in magenta. Ser16
and Thr30 are displayed in gold. The stereo-view of the best-fit
backbone superposition of 10 NMR derived structures selected
according to the lowest energy is shown in the Supplementary
Material. The structures are displayed with MOLMOL.[20] c:
Selected region of 2D NOESY experiments carried out in D[2]O,
showing a characteristic pattern of  -
NOEs
found in the WW fold. 1 corresponds to T30-V23, 2 to K12-F22, 3
to N24-W10, and 4 T14-V20. A table containing the proton
chemical shifts of the three domains is given as Supplementary
Material.
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