Figure 1.
FIG. 1. Arrhenius plots of the specificity constant s =
k[cat]/K[m] and k[cat] for the cleavage of FPR by thrombin (A)
and trypsin (B) wild-type ( ) and mutants G193A (
) and
G193P ( o ), in the temperature range from 5 °C to 45
°C. Note the use of the decimal logarithm in the ordinate.
Experimental conditions are as follows: 5 mM Tris, 0.1%
polyethylene glycol, 200 mM NaCl, pH 8.0. Continuous lines were
drawn according to Eqs. 4 and 5 in the text, with best fit
parameter values listed in Table II.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2005,
280,
25644-25650)
copyright 2005.
) and mutants G193A (
) and
G193P ( o ), in the temperature range from 5 °C to 45
°C. Note the use of the decimal logarithm in the ordinate.
Experimental conditions are as follows: 5 mM Tris, 0.1%
polyethylene glycol, 200 mM NaCl, pH 8.0. Continuous lines were
drawn according to Eqs. 4 and 5 in the text, with best fit
parameter values listed in Table II.