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Figure 1.
Fig. 1. Crystal structure of the substrate sensor DegS from
E. coli. (a) Schematic representation of DegS structure showing
the protease domain in brown and the PDZ domain in blue. N- and
C-termini (NT, CT), the β-strands (β1–β18), α-helices
(α1–α9) and loop structures (L1–L6) important for the
function are indicated. Residues of the catalytic triade and the
domain interface are highlighted and marked by dotted circles.
(b) Close-up of the 2|F[obs]−F[calc]| electron density map
calculated around the protease active center and contoured at
1.2σ. The residues forming the catalytic triade (His96, Asp126
and Ser201) are marked. (c) A close-up view of the
intramolecular hydrophilic contacts between protease (brown) and
PDZ (blue) domain. Important residues of the interface are
marked with numbers according to the DegS sequence.
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