Figure 1 - full size

Figure 1. Figure 1. Structure of PDZ45 tandem repeats of GRIP1. (a) Stereo view showing the backbone (N, C and C') of 20 superimposed NMR-derived structure of PDZ45 (residues 463 -658). The structures are superimposed against the averaged structure using residues 463 -658. Residues 463 -558 containing PDZ4 and the N-terminal extension are violet, residues 559 -568 encompassing the central linker are red and residues 569 -658 containing PDZ5 are blue. (b) Ribbon diagram of a representative NMR structure of PDZ45, colored as in panel a. The secondary structures of each PDZ domain are labeled following the standard scheme initially adopted by Doyle et al5. A prime following each secondary structure element (e.g., A') in PDZ5 is used to indicate the same secondary structure as in PDZ4. The -strand in the central linker is denoted as L. The two PDZ domains are related by a 32 Å translation and a 90° rotation around an axis roughly colinear with the direction of translation. (c) Molecular surface representation of PDZ45 showing the tight packing of the two domains into a supramodular structure. The surface is drawn according to its electric potential (red, negative; blue, positive). The molecule is drawn in the same orientation as in b.

The above figure is reprinted by permission from Macmillan Publishers Ltd: Nat Struct Biol (2003, 10, 972-978) copyright 2003.