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Figure 1.
FIG. 1. Structure of the SHANK1 PDZ domain. A, stereoview
of a ribbon diagram showing the monomeric structure of the Shank
PDZ-ligand complex. The  -strands are labeled
A-
F,
and the  -helices are labeled
A
and B. The ligand is
colored dark gray. The dotted line indicates a disordered loop
(residues 610-614) that is not seen in peptide-bound structure.
All of the residues in the loop were observed in peptide-free
structure. B, amino acid sequence alignment of the PDZ domains
from the rat and human Shank family, human NHERF (37), rat PSD95
(26), and rat GRIP1 (35). The sequences were aligned using the
program ClustalX (38). Highly conserved residues are shaded in
black and gray. The secondary structure elements of Shank1 PDZ
are shown as arrows ( -strands), bars ( -helices), and lines
(connecting loops). C, superposition of the PDZ domains. Black
ribbon indicates Shank1 PDZ domain. Light and dark gray ribbons
indicate PDZ domains of human NHERF (Protein Data Bank code 1G9O
[PDB]
) and rat PSD95 (Protein Data Bank code 1BFE [PDB]
), respectively.
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