Figure 1.
Figure 1. Characteristics of wild-type human PBGS relative to
the F12L variant. (a) The pH-rate profile for human PBGS (
)
exhibits a two-proton activating pK[a] of 5.9 and a one-proton
deactivating pK[b] of 8.3. In contrast, the F12L variant ( )
shows a single one-proton activating pK[a] of 8.5. (b) The
chromatographic separation of wild-type human (WT) PBGS and the
F12L variant on a mono-Q column. (c) The differential mobility
of wild-type (WT) human PBGS and the F12L variant on 12.5% (w/v)
native PAGE.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Biol
(2003,
10,
757-763)
copyright 2003.
)
exhibits a two-proton activating pK[a] of 5.9 and a one-proton
deactivating pK[b] of 8.3. In contrast, the F12L variant ( 
)
shows a single one-proton activating pK[a] of 8.5. (b) The
chromatographic separation of wild-type human (WT) PBGS and the
F12L variant on a mono-Q column. (c) The differential mobility
of wild-type (WT) human PBGS and the F12L variant on 12.5% (w/v)
native PAGE.