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Figure 1.
Figure 1. Ribbon diagrams of the complex and its components.
(a) Latent PAI-1 (ref. 9). In this structure, the reactive
center loop is inserted as strand 4 (red) of an expanded sheet A
(cyan), changing the shape of the binding site (yellow) for
somatomedin B. (b) The somatomedin B domain (gray with pink
disulfide bridges) binding to the region comprising helix E,
strand 1A and helix F (green) of active PAI-1. Dashed lines
indicate disordered residues in the reactive center loop (RCL)
of PAI-1 and residues leading to the RGD sequence of somatomedin
B. (c) The somatomedin B domain (blue at N terminus to red at C
terminus), showing disulfide bridges (pink) and side chains of
residues that interact with PAI-1. The sequence highlights
disulfide bridges (brackets), residues conserved among mammalian
vitronectin sequences (bold italic), residues with side chains
contacting PAI-1 (red) and the RGD motif (blue). (d) Comparison
of the active (green) and latent (yellow) conformations of PAI-1
showing how the shape of the binding surface changes upon
latency.
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