|
Figure 1.
Figure 1. The carbohydrate sequence attached at Asn297 of
human IgG1-Fc. Numbers attributes only sugars that have been
observed in the electron density. The sugars in bold belong to
the oligosaccharide core which is found in all naturally
occurring glycoforms, the addition of the other sugar residues
is variable. The biantennary mannose residues Man5 and Man8 that
are glycosidically linked to Man4 form the a(1-6) and a(1-3)
arms, respectively. A bisecting (GlcNAc)* occurs naturally in
5-10% of normal human IgG but not in IgG Cri used here. The
arrows point to the termini of respective glycoforms that have
been prepared by enzymatic truncation of native IgG-Fc
fragments. The native^** oligisaccharide is not fully
sialylated, but is a mixture of G0F, G1F, G2F and monosialylated
one.
|
