|
Figure 1.
Figure 1. Two αL I Domains Bound to an ICAM-1 Dimer(A)
Ribbon diagram of one monomeric unit of the
intermediate-affinity αL I domain (gold) complex with ICAM-1
domains 1-2 (cyan). The Mg^2+ ion is shown as a magenta sphere.
I domain MIDAS and ICAM-1 Glu-34 side chains are shown as
ball-and-stick with red oxygen atoms. The interacting β strands
C, D, and F of ICAM-1 are labeled. N-acetyl glucosamine residues
of ICAM-1 are shown with silver bonds.(B) The two ICAM-1-I
domain complexes in the crystallographic asymmetric unit are
shown with the I domains colored gold and the two ICAM-1
molecules colored cyan and green. The 2-fold axis between the
ICAM-1 molecules is in the vertical direction, normal to the
predicted membrane plane. Positions of the magnesium ions
(magenta spheres) and Glu-34 of ICAM-1 (CPK) are shown for
reference. The ICAM-1 Val-51 residues at the center of the
ICAM-1 dimer interface are shown as gray CPK models.(C) A view
of domains 1 of the ICAM-1 dimer (cyan and green), rotated about
90° from the view in (B), with domain 1 of the uncomplexed
ICAM-1 molecule A dimer (Casasnovas et al., 1998) superimposed
using domain 1 of one of the ICAM-1 molecules (black), whereas
the other one is colored gray. Figures 1–5 are prepared with
programs GLR (provided by L. Esser), Molscript (Kraulis, 1991),
Bobscript (Esnouf, 1997), Raster3D (Merritt and Murphy, 1994),
GRASP (Nicholls et al., 1991), and Povray (The Povray Team,
http://www.povray.org).
|
