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Figure 1.
Fig. 1. The HIF-1  destruction
sequence binds the  domain of
pVHL in an extended
strand-like conformation. (A) Schematic representation of the
15-residue portion of the HIF-1 destruction
sequence bound to the domain of
pVHL in the pVHL-ElonginB-ElonginC complex. The portion of HIF-1
that
adopts a continuous -strand
conformation is indicated by a wide arrow. HIF-1 is in
blue, Hyp564 in yellow, pVHL in red, ElonginB in magenta, and
ElonginC in green. C, COOH-terminus; N, NH[2]-terminus. The
figures are prepared with MOLSCRIPT (27), GL_RENDER, and POVRAY
(28). (B) Alignment of the first destruction sequence in the
ODDs of HIF-1 orthologs
and HIF-2 and HIF-3
paralogs,
highlighting residues identical in seven of the nine sequences.
The N and the C segments of human HIF-1 are
indicated in red. The putative N and C segments of the second
destruction sequences of HIF-1 and HIF-2
orthologs
are aligned below. The reported second destruction sequence is
38 residues long, and only a 23-residue region that aligns with
the first destruction sequence is shown. Dashes indicate gaps
relative to the seven-residue spacing between the putative N and
C segments of the second destruction sequence.
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