Figure 1.
Figure 1 Protein and DNA constructs used in the structure
determination. (A) The human VDR DBD. Sequence numbers are for
full-length hVDR and those in parentheses refer to the common
hormone receptor DBD numbering scheme. Residues in italics are
disordered in all of the structures. (B) The 18 bp DNA duplexes
used in co-crystallization, shown 5' arrow
3' in the top strand. Half-sites are shown in boxes and are
numbered by base pair. The DR3 sequence contains a direct repeat
of two consensus half-sites. SPP is the mouse osteopontin VDRE
and OC is the rat osteocalcin VDRE. Bases that differ from the
consensus sequence are shaded gray and the structurally
significant changes are highlighted in black. Estimates of
relative binding of VDR DBD homodimers to each sequence are also
shown.
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO J
(2002,
21,
2242-2252)
copyright 2002.
arrow
3' in the top strand. Half-sites are shown in boxes and are
numbered by base pair. The DR3 sequence contains a direct repeat
of two consensus half-sites. SPP is the mouse osteopontin VDRE
and OC is the rat osteocalcin VDRE. Bases that differ from the
consensus sequence are shaded gray and the structurally
significant changes are highlighted in black. Estimates of
relative binding of VDR DBD homodimers to each sequence are also
shown.