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Figure 1.
Fig. 1. Structure of the hNHERF PDZ1 domain bound to the
 [2]AR and
PDGFR carboxyl-terminal sequences. A, sequences of the chimeric
hNHERF PDZ1- [2]AR
(PDZ1B) and PDZ1-PDGFR (PDZ1P) proteins used in this study.
Amino acid residues belonging to the [2]AR and
PDGFR carboxyl termini are shaded in yellow. Secondary structure
elements are indicated at the top. B, stereo view of the hNHERF
PDZ1- [2]AR
crystal packing. Each carboxyl terminus serves as a ligand for a
neighboring PDZ1 molecule. C, ribbon diagram of the hNHERF PDZ1
domain bound to the [2]AR
carboxyl-terminal sequence NDSLL. A weighted 2F[obs]  F[calc]
electron density map calculated at 1.9-Å resolution and
contoured at 1.0  is
superimposed on the [2]AR
ligand. D, ribbon diagram of the hNHERF PDZ1 domain bound to the
PDGFR carboxyl-terminal sequence EDSFL. A weighted 2F[obs] F[calc]
electron density map calculated at 2.2-Å resolution and
contoured at 1.0 is
superimposed on the PDGFR ligand. The figure was made using
MOLSCRIPT (35), BOBSCRIPT (36), Raster3D (37), and POV-Ray
(www.povray.org).
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