Figure 1.
Figure 1. Schematic of the modified fragment assay. The
substrates are shown on the left. CCA-phenylalanine-caproic
acid-biotin (CCA-pcb) and C-puromycin (C-pmn) undergo a
ribosome-dependent reaction in which a peptide bond is formed
between the -amino
group of C-pmn and the carbonyl ester of the phenylalanine
moiety of CCA-pcb, yielding the two products:
C-puromycin-phenylalanine-caproic acid-biotin (C-pmn-pcb) and a
deacylated CCA.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Biol
(2002,
9,
225-230)
copyright 2002.
-amino
group of C-pmn and the carbonyl ester of the phenylalanine
moiety of CCA-pcb, yielding the two products:
C-puromycin-phenylalanine-caproic acid-biotin (C-pmn-pcb) and a
deacylated CCA.