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Figure 1.
Figure 1. Crystal Structure of P. furiosus RFCS(A) The
overall fold of an RFCS subunit is shown by a ribbon
representation. α helices and β strands are drawn by coils and
arrows, respectively. The chain is colored cyan for Domain 1,
green for Domain 2, and yellow for Domain 3. Walker A and B
motifs are colored red. ADP is shown as a purple stick model.(B)
The RFCS hexamer in the crystal is shown as a ribbon diagram
viewed from the N-terminal side. The six subunits are colored
blue, magenta, cyan, green, orange, and yellow-green for MolA to
MolF, respectively. The four ADP molecules bound to MolA, MolB,
MolC, and MolE are shown as red stick models.(C) A stereo view
of an omit F[o]-F[c] electron density map contoured at 3 σ
(ρ), drawn with ADP. Amino acid residues interacting with ADP
are depicted. Water molecules are indicated by white spheres.(D)
Orthogonal views of electrostatic potential mapped onto the
molecular surfaces as calculated by the program GRASP (Nicholls
and Honig, 1991). Positively charged surfaces are colored blue,
and negatively charged surfaces are red. The figure in the upper
left panel is viewed from the N-terminal side, as in (B). Trimer
1 (MolA to MolC) is indicated by a dotted line in the upper left
(N-terminal side) and upper right (C-terminal side) panels
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