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Figure 1.
Figure 1. (a) Overall structure of
KOD DNA polymerase. The struc-
ture is composed of domains and
subdomains, which are N-terminal
(N-ter, violet), Exonuclease (Exo,
blue), Polymerase (Pol) domain
including the Palm (brown) and
Fingers (green) subdomains and
the Thumb domain (red), including
the Thumb-1 and Thumb-2 subdo-
mains. Conserved carboxylate
residues in Polymerase and Exonu-
clease active site are shown by ball-
and-stick models. (b) Confor-
mational comparison of Thumb
domains among three archaeal
DNA polymerases. Red, KOD
DNA polymerase; blue, Tgo DNA
polymerase; and green, 9°N-7 DNA
polymerase. The comparison shows
that the Thumb domain of KOD
DNA polymerase displays the most
``opened'' conformation.
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