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Title
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Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12.
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Authors
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P.Freimuth,
K.Springer,
C.Berard,
J.Hainfeld,
M.Bewley,
J.Flanagan.
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Ref.
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J Virol, 1999,
73,
1392-1398.
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PubMed id
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Abstract
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The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is
predicted to consist of two immunoglobulin (Ig)-related structural domains. We
expressed the isolated CAR amino-terminal domain (D1) and a CAR fragment
containing both extracellular Ig domains (D1/D2) in Escherichia coli. Both D1
and D1/D2 formed complexes in vitro with the recombinant knob domain of
adenovirus type 12 (Ad12) fiber, and D1 inhibited adenovirus type 2 (Ad2)
infection of HeLa cells. These results indicate that the adenovirus-binding
activity of CAR is localized in the amino-terminal IgV-related domain and
confirm our earlier observation that Ad2 and Ad12 bind to the same cellular
receptor. Preliminary crystallization studies suggest that complexes of Ad12
knob bound to D1 will be suitable for structure determination.
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