Abstract for PubMed entry 958484

Title		Crystal structure of elastase-substrate complex at -- 55 degrees C.
Authors		T.Alber, G.A.Petsko, D.Tsernoglou.
Ref.		Nature, 1976, 263, 297-300.
PubMed id		958484
Abstract
The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.