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Title
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The use of electron tomography for structural analysis of disordered protein arrays.
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Authors
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K.A.Taylor,
J.Tang,
Y.Cheng,
H.Winkler.
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Ref.
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J Struct Biol, 1997,
120,
372-386.
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PubMed id
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Abstract
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A method based on Fourier transforms is described for obtaining a 3-D
reconstruction from a paracrystalline object with static disorder. The method is
derived from the standard methods used in 3-D reconstruction of 2-D crystals
except that all of the Fourier coefficients are used and not just the sampled
data from the periodic lattice. Thus, not only is the spatially ordered part of
the structure visualized in 3-D, but also the spatially disordered part.
Application of the method to 3-D reconstructions Application of the method to
3-D reconstructions of insect flight muscle is described as well as prospects
for extension of the method to radiation-sensitive specimens.
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