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Title
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Crystallization of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae.
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Authors
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A.F.Monzingo,
M.G.West,
E.Schelp,
D.R.Appling,
J.D.Robertus.
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Ref.
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Proteins, 1996,
26,
481-482.
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PubMed id
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Abstract
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Saccharomyces cerevisiae possesses three isozymes of
5,10-methylenetetrahydrofolate dehydrogenase (MTD). The NAD-dependent enzyme is
the first monofunctional form found in eukaryotes. Here we report its
crystallization in a form suitable for high-resolution structure. The space
group is P4(2)2(1)2 with cell constants a = b = 75.9, c = 160.0 A, and there is
one 36 kDa molecule in the asymmetric unit. Crystals diffract to 2.9 A
resolution.
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