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Title
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Identification of a DinB/UmuC homolog in the archeon Sulfolobus solfataricus.
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Authors
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O.I.Kulaeva,
E.V.Koonin,
J.P.McDonald,
S.K.Randall,
N.Rabinovich,
J.F.Connaughton,
A.S.Levine,
R.Woodgate.
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Ref.
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Mutat Res, 1996,
357,
245-253.
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PubMed id
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Abstract
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To date, eight closely related homologs of the Escherichia coli UmuC protein
have been identified. All of these homologs appear to play critical roles in
damage-inducible mutagenesis in enterobacteriaceae. Recently, a distantly
related UmuC-homolog, DinB, has also been identified in E. coli. Using the
polymerase chain reaction together with degenerate primers designed against
conserved regions found in UmuC-like proteins, we have identified a new member
of the UmuC-superfamily in the archeon Sulfolobus solfataricus. This new homolog
shows high sequence similarity to DinB and a lower level of similarity to UmuC.
As a consequence, we have called this new gene dbh (dinB homolog). Analysis of
approximately 2.7 kb DNA encompassing the dbh region revealed several open
reading frames (orfs). One, encoding a putative ribokinase, was located
immediately upstream of dbh. This orf overlaps the dbh gene by 4 bp suggesting
that both proteins might be coordinately expressed. Further upstream of the
ribokinase-dbh locus was another orf encoding a potential ATPase homologous to
two uncharacterized S. cerevisiae proteins (YD9346.02c and SC38KCXVI_20) and
another E. coli DNA repair protein, RuvB. While this is the first report of a
UmuC-like homolog in an archeon, we detected additional homologs using protein
sequence comparisons in Gram-positive bacteria, cyanobacteria, and among
potential human EST products, indicating that UmuC-related proteins comprise a
ubiquitous superfamily of proteins probably involved in DNA repair and
mutagenesis.
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