Abstract for PubMed entry 8732

Title		Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures.
Authors		A.L.Fink, A.I.Ahmed.
Ref.		Nature, 1976, 263, 294-297.
PubMed id		8732
Abstract
Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.