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A single-site human growth hormone mutant (hGH[G120R]), which inhibits receptor
dimerization, was used to produce single crystals, suitable for high-resolution
diffraction studies, of 1:1 complexes with the ligand-binding domain of the
growth hormone receptor (hGHbp) and of the prolactin receptor (hPRLbp). Crystals
of the hGH[G120R]-hGHbp complex are in space group P4(1)2(1)2 or P4(3)2(1)2 with
a = 67.7 A, c = 228.0 A, and diffract to at least 2.2 A. Crystals of the complex
between hGH[G120R] and hPRLbp are in space group P2(1)2(1)2 with a = 154.0 A, b
= 68.4 A, c = 42.9 A, and diffract to at least 2.8 A. The structures of these
two complexes will shed light on the early events in receptor activation, and
provide the basis for an analysis of receptor specificity of growth hormone and
prolactin.
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