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Title
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Purification and crystallization of human cyclin-dependent kinase 2.
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Authors
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J.Rosenblatt,
H.De Bondt,
J.Jancarik,
D.O.Morgan,
S.H.Kim.
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Ref.
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J Mol Biol, 1993,
230,
1317-1319.
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PubMed id
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Abstract
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The major transitions of the eukaryotic cell cycle are triggered by
cyclin-dependent protein kinases. We report the purification and crystallization
of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has
been implicated in the control of the G1/S transition. CDK2 was purified in
large quantities from insect cells infected with a recombinant baculovirus, and
crystals of the protein were prepared and subjected to preliminary X-ray
diffraction analysis.
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