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Title
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Crystallization and preliminary X-ray diffraction studies of the soluble 14 kDa beta-galactoside-binding lectin from bovine heart.
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Authors
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Y.Bourne,
B.Bolgiano,
M.P.Nésa,
P.Penfold,
D.Johnson,
T.Feizi,
C.Cambillau.
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Ref.
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J Mol Biol, 1994,
235,
787-789.
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PubMed id
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Abstract
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The soluble 14 kDa beta-galactoside-binding lectin from bovine heart, a member
of the S-type lectin family, has been crystallized in a form suitable for X-ray
diffraction analysis. The crystals, in the absence of a saccharide ligand,
diffract beyond 2.5 A resolution. They are obtained from polyethylene glycol
6000 at pH 6.0. Crystals grow as monoclinic plates, space group P2(1), with cell
dimensions: a = 35.47 A, b = 64.33 A, c = 58.78 A and beta = 91.7 degrees. The
asymmetric unit contains two molecules related by a 2-fold non-crystallographic
axis. Two lectin monomers in the asymmetric unit give a Vm of 2.4 A3/Da, i.e. a
solvent content of approximately 50%. The complex of lectin with the saccharide
ligand, N-acetyllactosamine, crystallizes in the space group P2(1)2(1)2 with
cell dimensions: a = 63.55 A, b = 82.13 A and c = 62.39 A. Crystals of this
complex diffract beyond 2.0 A resolution. Two complexes in the asymmetric unit
lead to a Vm value of 2.8 A3/Da (57% solvent).
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