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Title
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Structure of the regulatory domain of scallop myosin at 2.8 A resolution.
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Authors
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X.Xie,
D.H.Harrison,
I.Schlichting,
R.M.Sweet,
V.N.Kalabokis,
A.G.Szent-Györgyi,
C.Cohen.
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Ref.
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Nature, 1994,
368,
306-312.
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PubMed id
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Abstract
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The regulatory domain of scallop myosin is a three-chain protein complex that
switches on this motor in response to Ca2+ binding. This domain has been
crystallized and the structure solved to 2.8 A resolution. Side-chain
interactions link the two light chains in tandem to adjacent segments of the
heavy chain bearing the IQ-sequence motif. The Ca(2+)-binding site is a novel
EF-hand motif on the essential light chain and is stabilized by linkages
involving the heavy chain and both light chains, accounting for the requirement
of all three chains for Ca2+ binding and regulation in the intact myosin
molecule.
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