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Title
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Secondary structure of the oct-3 POU homeodomain as determined by 1H-15N NMR spectroscopy.
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Authors
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E.H.Morita,
M.Shirakawa,
F.Hayashi,
M.Imagawa,
Y.Kyogoku.
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Ref.
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FEBS Lett, 1993,
321,
107-110.
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PubMed id
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Abstract
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Most of the 1H and 15N magnetic resonances of the 66 amino acid long POU
homeodomain of mouse Oct-3 have been assigned by the combined use of the
two-dimensional homonuclear, and two- and three-dimensional heteronuclear NMR
methods. The sequential NOE connectivities and amide proton exchange
measurements indicate the presence of three helical regions within the domain.
The positions of the three helices correspond well to those of other
homeodomains, the three-dimensional structures of which have already been
determined. The present NMR study provides the first experimental evidence for
the existence of a helix-turn-helix motif in the oct-3 POU homeodomain.
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