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Title
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Characterization of two crystal forms of Clostridium thermocellum endoglucanase CelC.
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Authors
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R.Dominguez,
H.Souchon,
P.M.Alzari.
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Ref.
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Proteins, 1994,
19,
158-160.
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PubMed id
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Abstract
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Endoglucanase CelC from Clostridium thermocellum expressed in Escherichia coli
has been crystallized in two different crystal forms by the hanging drop method.
Crystals of form I were grown with polyethylene glycol as a precipitant. They
are orthorhombic, space group P2(1)2(1)2(1), with cell dimensions a = 51.4 A, b
= 84.3 A, and c = 87.5 A. Crystals of form II, obtained in ammonium sulfate
solutions, belong to the tetragonal space group P4(1)2(1)2 (or P4(3)2(1)2) with
cell dimensions of a = b = 130.7 A and c = 69.6 A. Diffraction data to 2.8 A
resolution were observed for both crystal forms with a rotating anode generator.
Preliminary oscillation images of the orthorhombic form I crystals using a
synchrotron radiation source show diffraction to 2.2 A resolution, indicating
that these crystals are suitable for high resolution crystallographic analysis.
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