 |
|
Title
|
|
Crystallization and preliminary crystallographic study of human CksHs1: a cell cycle regulatory protein.
|
|
|
Authors
|
|
A.S.Arvai,
Y.Bourne,
D.Williams,
S.I.Reed,
J.A.Tainer.
|
|
|
Ref.
|
|
Proteins, 1995,
21,
70-73.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The cell cycle regulatory protein CksHs1 has been crystallized in a form
suitable for X-ray studies. CksHs1 crystals were grown in the presence of
vanadate, a phosphatase inhibitor, but were also obtained with phosphate or
tungstate as a cofactor. They belong to the hexagonal space group P6(1)22 with
unit cell dimensions: a = b = 94 A, c = 131.6 A, and gamma = 120 degrees. The
crystals grown in the presence of vanadate diffract X-rays to at least 2.8 A.
Molecular replacement results from the homologous human CksHs2 structure reveal
that a dimer forms the crystal habit, giving the unusual Vm value of 4.4 A3/Da
or a solvent content of 72%.
|
|
|
|