 |
|
Title
|
|
X-ray diffraction studies of troponin-C crystals from rabbit and chicken skeletal muscles.
|
|
|
Authors
|
|
G.M.Strasburg,
M.L.Greaser,
M.Sundaralingam.
|
|
|
Ref.
|
|
J Biol Chem, 1980,
255,
3806-3808.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Crystals of troponin-C from rabbit and chicken muscles have been obtained using
the vapor diffusion method. Crystals of rabbit troponin-C are tetragonal with a
unit cell of a = b = 89.4 A, c = 79.9 A, space group P42, and 4
molecules/asymmetric unit. Diffraction from these crystals is limited to 7A and
the crystals are unstable under x-ray irradiation, making them unsuitable for a
high resolution structural determination. Crystals of chicken troponin-C are
trigonal with a unit cell of a = b = 66.7 A, c = 60.8 A. The space group is
P3121 or P3221 and there is only 1 molecule/asymmetric unit. These crystals
diffract to at least 2.2 A, are very stable under x-ray irradiation, and are
suitable for high resolution study.
|
|
|
|