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Title
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Complete amino acid sequences of two protease inhibitors in the venom of Bungarus fasciatus.
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Authors
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C.S.Liu,
T.C.Wu,
T.B.Lo.
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Ref.
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Int J Pept Protein Res, 1983,
21,
209-215.
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PubMed id
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Abstract
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Two analogous protease inhibitors, VIIIb and IX in the venom of Bungarus
fasciatus were reduced and carboxymethylated. Tryptic peptides were separated by
cellulose thin-layer peptide mapping technique, and amino acid sequences were
analyzed by DABITC/PITC double coupling method. Alignment of all tryptic
peptides was established by analyses of chymotryptic peptides and further
confirmed by Staphylococcus aureus V8 protease digestion. IX consisted of 65
amino acid residues. VIIIb consisted of 62 residues, identical to the N-terminal
62-amino acid sequence of IX.
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