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Title
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Crystallization of the fab fragments of monoclonal anti-p-azophenylarsonate antibodies and their complexes with haptens.
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Authors
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R.A.Mariuzza,
A.G.Amit,
G.Boulot,
P.Saludjian,
F.A.Saul,
P.Tougard,
R.J.Poljak,
J.Conger,
E.Lamoyi,
A.Nisonoff.
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Ref.
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J Biol Chem, 1984,
259,
5954-5958.
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PubMed id
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Abstract
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We report on the preparation, crystallization, and preliminary x-ray
crystallographic study of Fab fragments from monoclonal anti-p-azophenylarsonate
antibodies. Several crystalline forms were obtained with the Fab fragment from
the R19.9 monoclonal antibody as well as with the complex between the hapten
p-aminobenzenearsonic acid and Fab R19.9. The crystals of this hapten-Fab
complex are similar to but not always isomorphous with the native Fab crystals.
All the native and complex crystals were obtained using polyethylene glycol 6000
as crystallizing agent. Some of these crystalline forms diffract to a 2-A
resolution or beyond and are suitable for high resolution x-ray diffraction
analysis. A possible interpretation of hapten binding to crystalline Fab
fragments from R19.9 and from the R9.3 monoclonal anti-p-azophenylarsonate
antibody, implying conformational changes, is discussed.
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